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By Ben Dawes (ed.)

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Example text

Clearly, incidence of parasitaemia may be affected also by the characteristic reaction of a given host species to infection. Whether parasitaemia in an animal species is a reliable guide to the propensity of that species to infect Glossina is equally uncertain. Microscopically recognizable parasitaemia is not a prerequisite for the infection of Glossinu and wide differences may exist in the proportion of flies becoming infected by feeding on different animals with apparently similar parasitaemias (examples in Lumsden, 1962).

H. R. LUMSDEN then incubating the cultures at 30-32” C. Trager considered that the success of the cultures was related to their being incubated at that temperature rather than the usual one of 28” C. Very profuse trypanosome multiplication was obtained and the insect tissue appeared to be unharmed by the presence of the organisms. Trager made several attempts to infect sheep with the culture trypanosomes and succeeded on two occasions, with trypanosomes which had been maintained in vitro for 39 and 16 days, respectively.

BIOLOGICAL ASPECTS OF TRYPANOSOMIASIS RESEARCH 11 necessarily left uncontrolled were likely to be of negligible effect. Initial assessment of the usefulness of the method depended on demonstration that it yielded results which were consistent and of such precision, in relation to the total range of variation occurring, that differences between estimates might be recognized as significant. , 1963). No statistically significant differences occurred among the ten estimates and the estimates were of such precision, using tenfold dilution intervals and six recipient animals at each dilution, that differences of about tenfold and upward could be recognized as significant.

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