By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the problematic paintings of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – hide various issues in pluripotent stem mobilephone culturing together with mouse and human fibroblasts, human embryonic stem cells and caused pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in Culture is a entire selection of illustrated suggestions complemented through informative and academic captions analyzing what high quality cells appear like and the way they behave in numerous environments. Examples of ideal cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and triggered pluripotent stem cellphone colonies. This precise and thorough atlas is a useful source for researchers, academics, and scholars who're attracted to or operating with stem cellphone culturing.
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Cell Transplant. 2010;19:509–23. Lee JB, Song JM, Lee JE, Park JH, Kim SJ, Kang SM, et al. Available human feeder cells for the maintenance of human embryonic stem cells. Reproduction. 2004;128:727–35. Leonardo TR, Schell JP, Tran HT, Peterson SE. Preparation of mouse embryonic fibroblast feeder cells. In: Loring JF, Peterson SE, 3 Inactivated Mouse and Human Fibroblasts editors. Human stem cell manual. 2nd ed. London: Elsevier; 2012. p. 15–27. Michalska AE. Isolation and propagation of mouse embryonic fibroblasts and preparation of mouse embryonic feeder layer cells.
In: Amit M, Itskovitz-Eldor J, editors. Atlas of human pluripotent stem cells: derivation and culturing, Stem cell biology and regenerative medicine. New York: Humana Press; 2012. p. 15–39. Diekmann U, Spindler R, Wolkers WF, Glasmacher B, Müller T. Cryopreservation and quality control of mouse embryonic feeder cells. Cryobiology. 2011;63:104–10. Eiselleova L, Peterkova I, Neradil J, Slaninova I, Hampl A, Dvorak P. Comparative study of mouse and human feeder cells for human embryonic stem cells.
B) The same culture of cells is confluent by 96 h post-passaging. Note that a few apoptotic cells (small round cells) are starting to appear in the culture (Both ×4 magnification) 18 a 2 Mouse and Human Fibroblasts b c Fig. 22 (a–c) Three different human fibroblast cultures, all confluent (×4 magnification). These cells will no longer be proliferating because there is no space for new cells to occupy after mitotic cell division. (a, b) Overconfluent cells; a large number of apoptotic cells can be identified.