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By Nader Hassanzadeh

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Coli as a normal phage. It is a temperate phage so it has both lytic and lysogenic growth cycles. The Mu prophage integrates into the host genome. However, it does not do so by a site-specific recombination rather, it integrates randomly into the host genome by a transpositional mechanism. When Mu replicates, it does so by replicative transposition events in which each new copy of Mu is inserted somewhere else in the chromosome. Mu is, in effect, a transposon that happens to have genes that package its DNA as well as genes that regulate its transposition.

Transposable elements   Transposons Insertion sequences (IS) Transposable elements have become valuable mutagenic tools for genetic and molecular analysis in many different bacteria. 39 Transposable genetic elements in Bacteria    1. 2. 3. 4. Mobile genetic elements were discovered in bacteria in the late 1960s. They were also recognized because they caused a particular type of mutation which was unstable. We can recognize four types of bacterial transposon: Insertion Sequences Transposons Composite Transposons Bacteriophage Elements Martin E.

Insertion sequences are short (600-1500 base pairs long) simple transposons that do not carry genes beyond those essential for insertion of the transposon into E. coli. Complex transposons are much larger and carry additional genes. 58 Four modes of transposition 59 Mechanisms of Transposition  1. 2. There are two mechanisms by which transposons move: Simple transposition, Replicative transposition 60 Simple transposition Direct or conservative transposition      Also known as direct or conservative transposition involves the transfer of the element from one place to another.

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