By Lihua Xiao, Una Ryan, Yaoyu Feng
While a couple of introductory books on uncomplicated and molecular biology can be found, none spotlight the foodborne parasitic pathogens. previously. A cutting-edge assessment, Biology of Foodborne Parasites charts major growth and descriptions key organic options utilized to foodborne parasitic pathogens examine. The ebook covers easy biology, genetics and genomics, epidemiology, pathogenesis, prognosis, keep an eye on, and prevention. It showcases fresh learn that may then be used to spark extra breakthroughs.
The booklet addresses difficult matters in foodstuff pathogen detection. It details person foodborne protists and helminthes, with every one bankruptcy following the same structure for a constant presentation of knowledge. It discusses issues starting from simple biology, genetics and genomics, molecular detection and typing, and pathogenesis to epidemiology, molecular epidemiology, therapy and prevention, between different present matters. It additionally info the equipment used to diagnose the an infection, represent the pathogen, and notice parasites in 3 nutrients commodities: meats, water, and clean produce.
With chapters written by means of specialists of their respective fields, the e-book offers a competent roadmap for destiny improvement of more suitable, leading edge organic and molecular tools for research of foodborne parasitic pathogens. A convenient, complete reference on all elements of biology of foodborne parasites, it highlights learn wishes and instructions, aiding you increase complex diagnostic instruments and new intervention measures.
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Extra info for Biology of foodborne parasites
Immunoassays have benefits of technical simplicity, rapidity, and cost-effectiveness. Latex particle agglutination and coagglutination tests, enzyme-linked immunoassays, and direct immunofluorescence antibody assays have been available for some years. 12 Finally, an important limitation of all conventional diagnostic methods is their inability to differentiate between different “strains” of the same pathogen. 1 Polymerase Chain Reaction The advent of molecular tools, particularly those based on the polymerase chain reaction (PCR), has provided a major advance for the food industry because of the ability to detect low levels of pathogens on food.
M. , Waterborne protozoan pathogens. Clin. Microbiol. , 10, 67, 1997. 19. Gonin, P. , Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay. J. Clin. , 41, 237, 2003. 20. S. , Amoebiasis. Mayo Clin. , 83, 1154, 2008. M. , Immune response to Giardia infection: Lessons from animal models. In: Giardia and Cryptosporidium: From Molecules to Disease, Ortega Pierres, G. , eds. CABI, Cambridge, MA, p. 451, 2009.
5 Microarray Detection DNA microarray technology has also been explored as a detection tool for foodborne pathogens. A DNA microarray can contain several thousand surface-immobilized DNA probes on a small glass slide. Following hybridization of target DNA sequences to probes anchored on the chip surface, fluorescence detection is used to monitor binding events. 60 More recently, a random genomic DNA microarray for the detection and identification of Listeria monocytogenes in milk was developed with a detection limit of log CFU/mL of L.