Download Cholesterol and phytosterol oxidation products : analysis, by Francesc Guardiola PDF

By Francesc Guardiola

Content material: ldl cholesterol Oxidation items 1. ldl cholesterol Oxidation Mechanisms 2. Extraction and Purification of ldl cholesterol Oxidation items three. choice of ldl cholesterol Oxidation items by means of gasoline Chromatography four. selection of ldl cholesterol Oxidation items by means of High-Performance Liquid Chromatography five. decision of ldl cholesterol Oxidation items through Thin-Layer Chromatography 6. Harmonization of ldl cholesterol Oxidation Product research 7. Formation and content material of ldl cholesterol Oxidation items in Egg and Egg items eight. Formation and content material of ldl cholesterol Oxidation items in Milk and Dairy items nine. Formation and content material of ldl cholesterol Oxidation items in Meat and Meat items 10. Formation and content material of ldl cholesterol Oxidation items in Seafood and Seafood items eleven. Formation and content material of ldl cholesterol Oxidation items in different meals 12. foundation and content material of ldl cholesterol Oxidation items in organic Samples thirteen. ldl cholesterol Oxidation items and Atherosclerosis 14. ldl cholesterol Oxidation items: different organic results Phytosterol Oxidation items 15. Formation and content material of Phytosterol Oxidation items in meals sixteen. selection of Phytosterol Oxidation items in meals and organic Samples 17. organic results of Phytosterol Oxidation items, destiny study components and Concluding feedback

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Extra resources for Cholesterol and phytosterol oxidation products : analysis, occurrence, and biological effects

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Artifact Formation Cholesterol is vulnerable to oxidation in the presence of air, light, and suitable catalysts. Therefore, it is advisable to take precautions to exclude these factors as much as possible and to remove endogenous cholesterol from the sample as early as possible in the analytical chain. However, some artifactual cholesterol oxidation during sample preparation seems to be unavoidable and difficult to control. Radiotracer technology has been used as a very sensitive tool to determine the level of artifactual COP formation during sample preparation.

Nevertheless, spurious peaks were seen in the total ion chromatogram when separating the silylated extract by GLCMS. The remaining procedures (lanes 4, 6, 9, and 10), although efficient in removing the bulk of triacylglycerol, left traces of cholesterol and/or partial acylglycerols Copyright © 2002 by AOCS Press. Fig. 6. Efficiency of several solid-phase extraction (SPE) procedures for the purification of cholesterol oxidation products (COP). 1 COP standard solution; 2 lipid standard (cholesteryl oleate, triolein, oleic acid, and cholesterol); 3 phosphatidylcholine; 4 silica (Si)-SPE (36); 5 octadecyl-modified silica (ODS)-SPE (64); 6 aminopropyl-modified silica (NH2)-SPE (61); 7 Si-SPE (7); 8 Si-SPE (31); 9 NH2- plus ODS-SPE (58); 10 NH2-SPE (66).

Using a silica SPE cartridge and the elution scheme devised by Lai et al. (31) produced the cleanest extract (lane 8). Nevertheless, spurious peaks were seen in the total ion chromatogram when separating the silylated extract by GLCMS. The remaining procedures (lanes 4, 6, 9, and 10), although efficient in removing the bulk of triacylglycerol, left traces of cholesterol and/or partial acylglycerols Copyright © 2002 by AOCS Press. Fig. 6. Efficiency of several solid-phase extraction (SPE) procedures for the purification of cholesterol oxidation products (COP).

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