By John G. Day, Mark R. McLellan
This ebook presents distinctive protocols for all of the most modern methodologies used to guarantee the long term biostorage of a various diversity of organic fabrics.
built in specialist laboratories, the protocols were painstakingly perfected through the years to supply time-tested, step by step directions that make certain strong and reproducible effects. each one protocol offers with the maintenance of an organelle, telephone, or tissue sort, and is offered even to the nonspecialist as a result of its cookbook method. Novel protocols will be with ease constructed with the aid of the "hands-on" Notes sections.
Cryopreservation and Freeze-Drying Protocols is an quintessential reference paintings for either the person researcher, and all those that are looking to determine or increase biostorage platforms of their laboratories. Its purposes variety from microbial tradition collections, botanic gardens, and zoos to animal husbandry, aquaculture, medication, human fertilization, and phone and molecular biology.
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Extra info for Cryopreservation and Freeze-Drying Protocols
Cell densities of more than lO%nL are commonly used (3). 4. 2-n& aliquots into sterile ampules using a sterile syringe with long needle or a sterile long Pasteur pipet as in step 3 (see Note 15), avoid touching the inside or top of the ampules (see Note 16). Plug the ampules with sterile cotton wool. 5. Directly immerse the ampules in a cold bath and qmckly stir for = 10 s to ensure that cells are spread on the inner surface of ampules (seeNotes 17-19). Leave the ampules standing in the cold bath to freeze for more than 2 min.
J. (1990) Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiology 27,219-23 1. 6. Kirsop, B. E. and Doyle, A. , Academic, London. 7. Rudge, R. H. (1991) Maintenance of bacteria by freeze-drying, in Maintenance of Macro-Organisms and Cultured Cells: A Manual of Laboratory Methods, 2d ed. (Kirsop, B. E. ), Academic, London, pp. 31-44. 8 Heckly, R. J. (1978) Preservation of micro-organisms. Adv. Appl. Microb. 3,1-76.
Mazur, P. (1977) The role of intracellular freezing in the death of cells cooled at supraoptimal rates. Cryobiology 14,25 l-272. CHAPTER4 Freeze-Drying of Yeasts Sugio Kawamura, Yukie Murakami, Yukie Miyamoto, and Kazuo Kimura 1. Introduction Increased interest in biotechnology along with the requirement for strains associated with patent applications to be deposited in a recognized collection, under the terms of the Budapest Treaty (1977), has increased the demand for the maintenance of microorganisms in desig- nated culture collections.